Part:BBa_K3726072:Design
Lv.1 AcrbV2 - C
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 896
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 436
Illegal NheI site found at 459
Illegal NheI site found at 625
Illegal PstI site found at 896 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 896
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 896
Illegal NgoMIV site found at 2032
Illegal NgoMIV site found at 3548
Illegal AgeI site found at 109 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is a MoClo Lv.1 part, assembled in the acceptor Lv1 entry vector “BBa_K3228069” P1_pANs_SpecR, which is a self replicating shuttle vector. This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by two connectors that will allow the assembly of a Lv2 construct. In its upstream region the connector is 5'Con2 “BBa_K2560066”, and in its downstream region 3'Con2 “BBa_K2560071”.
This design will allow us to test which “tolerance and secretion ” variant has better efficiency by its own account as a replicative vector, and then assemble them as an integrative Lv2 Moclo construct. To know more about this, visit our webpage: https://2021.igem.org/Team:MADRID_UCM/Design
Source
This construct has been made by golden gate reaction.
References
"Enhancing Tolerance to Short-Chain Alcohols by Engineering the Escherichia coli AcrB Efflux Pump to Secrete the Non-native Substrate n-Butanol", ACS Publications, 2021. [Online]. Available: https://pubs.acs.org/doi/10.1021/sb400065q.